Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production
Additional file 1: Assembled transcripts for sample OP1 less than 200 base pairs in length. (782.6Kb)
Additional file 2: Assembled transcripts for sample OP2 less than 200 base pairs in length. (1.095Mb)
Additional file 3: Assembled transcripts for sample CP1 less than 200 base pairs in length. (1.380Mb)
Additional file 4: Assembled transcripts for sample CP2 less than 200 base pairs in length. (1.467Mb)
Additional file 9: GO term assignments, BLAST identity, and expression analysis for genes with significant differential expression in Cuffdiff or edgeR between OP and CP B. calyciflorus. (331.3Kb)
Additional file 11: Housekeeping, meiosis inventory, histone, and dormancy genes identified in OP and CP libraries. (173.7Kb)
Additional file 12: Nuclear receptor superfamily members identified in OP and CP libraries. (33.34Kb)
Hanson, Sara J.
Mark Welch, David B.
Logsdon, John M.
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KeywordEvolution of sexual reproduction; Differential expression analysis; Gene ontology analysis; Meiosis; Gametogenesis; Resting eggs; Mixis induction
Sexual reproduction is a widely studied biological process because it is critically important to the genetics, evolution, and ecology of eukaryotes. Despite decades of study on this topic, no comprehensive explanation has been accepted that explains the evolutionary forces underlying its prevalence and persistence in nature. Monogonont rotifers offer a useful system for experimental studies relating to the evolution of sexual reproduction due to their rapid reproductive rate and close relationship to the putatively ancient asexual bdelloid rotifers. However, little is known about the molecular underpinnings of sex in any rotifer species. We generated mRNA-seq libraries for obligate parthenogenetic (OP) and cyclical parthenogenetic (CP) strains of the monogonont rotifer, Brachionus calyciflorus, to identify genes specific to both modes of reproduction. Our differential expression analysis identified receptors with putative roles in signaling pathways responsible for the transition from asexual to sexual reproduction. Differential expression of a specific copy of the duplicated cell cycle regulatory gene CDC20 and specific copies of histone H2A suggest that such duplications may underlie the phenotypic plasticity required for reproductive mode switch in monogononts. We further identified differential expression of genes involved in the formation of resting eggs, a process linked exclusively to sex in this species. Finally, we identified transcripts from the bdelloid rotifer Adineta ricciae that have significant sequence similarity to genes with higher expression in CP strains of B. calyciflorus. Our analysis of global gene expression differences between facultatively sexual and exclusively asexual populations of B. calyciflorus provides insights into the molecular nature of sexual reproduction in rotifers. Furthermore, our results offer insight into the evolution of obligate asexuality in bdelloid rotifers and provide indicators important for the use of monogononts as a model system for investigating the evolution of sexual reproduction.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in BMC Genomics 14 (2013): 412, doi:10.1186/1471-2164-14-412.
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