Characterization of new microsatellite loci for population genetic studies in the Smooth Cauliflower Coral (Stylophora sp.)
Berumen, Michael L.
Voolstra, Christian R.
MetadataShow full item record
A total of one hundred microsatellites loci were selected from the draft genome of Stylophora pistillata and evaluated in previously characterized samples of Stylophora cf pistillata from the Red Sea. 17 loci were amplified successfully and tested in 24 individuals from samples belonging to a single population from the central region of the Red Sea. The number of alleles ranged from 3 to 15 alleles per locus, while observed heterozygosity ranged from 0.292 to 0.95. Six of these loci showed significant deviations from Hardy–Weinberg equilibrium (HWE) expectations, and 4/136 paired loci comparisons suggested linkage disequilibrium after Bonferroni corrections. After excluding loci with significant HWE deviation and evidence of null alleles, average genetic diversity over loci in the population studied (N = 24, Nloci = 11) was 0.701 ± 0.380. This indicates that these loci can be used effectively to evaluate genetic diversity and undertake population genetics studies in Stylophora sp. populations.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Conservation Genetics Resources 5 (2013): 561-563, doi:10.1007/s12686-012-9852-x.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Kin and population recognition in sympatric Lake Constance perch (Perca fluviatilis L.) : can assortative shoaling drive population divergence? Behrmann-Godel, Jasminca; Gerlach, Gabriele; Eckmann, Reiner (2005-08-02)Prior studies have shown that perch (Perca fluviatilis L.) of Lake Constance belong to two genetically different but sympatric populations, and that local aggregations of juveniles and adults contain closely related kin. ...
Development of microsatellite markers in the toxic dinoflagellate Alexandrium minutum (Dinophyceae) Nagai, Satoshi; McCauley, Linda A. R.; Yasuda, N.; Erdner, Deana L.; Kulis, David M.; Matsuyama, Y.; Itakura, S.; Anderson, Donald M. (2006-01-22)Outbreaks of paralytic shellfish poisoning caused by the toxic dinoflagellate Alexandrium minutum (Dinophyceae) are a worldwide concern from both the economic and human health points of view. For population genetic studies ...
Estimating connectivity in marine populations : an empirical evaluation of assignment tests and parentage analysis under different gene flow scenarios Saenz-Agudelo, Pablo; Jones, Geoffrey P.; Thorrold, Simon R.; Planes, Serge (2008-11-21)The application of spatially explicit models of population dynamics to fisheries management and the design marine reserves network systems has been limited due to a lack of empirical estimates of larval dispersal. Here we ...