An evaluation of staining techniques for marking daily growth in scleractinian corals
Cohen, Anne L.
McCorkle, Daniel C.
MetadataShow full item record
In situ skeletal markers have been widely used to quantify skeletal growth rates of scleractinian corals on sub-annual time-scales. Nevertheless, an evaluation of different techniques, both in terms of their efficacy and potential impacts on the growth process itself, has not been undertaken. Here the effects of exposure to four different dyes (alizarin, alizarin complexone, calcein, oxytetracycline) and isotope spikes (Ba and Sr) on the growth rates of scleractinian corals are compared. Oxytetracycline increased coral growth. Alizarin, alizarin complexone, calcein, and Sr and Ba isotope spikes had no significant effect on coral growth, but polyp extension appeared reduced during exposure to alizarin and alizarin complexone. Calcein provided a more intense fluorescent mark than either alizarin or alizarin complexone. Isotope spikes were challenging to locate using isotope ratio analysis techniques. Thus, calcein appears best suited for marking short-term calcification increments in corals, while a combination of alizarin or alizarin complexone and calcein may be useful for dual labeling experiments as there is little overlap in their fluorescence spectra.
Author Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Journal of Experimental Marine Biology and Ecology 440 (2013): 126-131, doi:10.1016/j.jembe.2012.12.003.
Showing items related by title, author, creator and subject.
Coral biomineralization, climate proxies and the sensitivity of coral reefs to CO2-driven climate change DeCarlo, Thomas M. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2017-02)Scleractinian corals extract calcium (Ca2+) and carbonate (CO2−3) ions from seawater to construct their calcium carbonate (CaCO3) skeletons. Key to the coral biomineralization process is the active elevation of the CO2−3 ...
Coral calcification : insights from inorganic experiments and coral responses to environmental variables Holcomb, Michael (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 2010-02)Coral calcification is examined using a laboratory model and living corals. In the laboratory model, abiogenic aragonite formed at high saturation state (Ω>~20) had a granular appearance and was enriched in trace elements, ...
Comment on "Radiocarbon calibration curve spanning 0 to 50,000 years BP based on paired 230Th/234U/238U and 14C dates on pristine corals" by R.G. Fairbanks et al. (Quaternary Science Reviews 24 (2005) 1781-1796), and "Extending the radiocarbon calibration beyond 26,000 years before present using fossil corals" by T.-C. Chiu et al. (Quaternary Science Reviews 24 (2005) 1797-1808). Reimer, Paula J.; Baillie, Mike G. L.; Bard, Edouard; Beck, J. Warren; Blackwell, Paul G.; Buck, Caitlin E.; Burr, George S.; Edwards, R. Lawrence; Friedrich, Michael; Guilderson, Thomas P.; Hogg, Alan G.; Hughen, Konrad A.; Kromer, Bernd; McCormac, Gerry; Manning, Sturt; Reimer, Ron W.; Southon, John R.; Stuiver, Minze; van der Plicht, Johannes; Weyhenmeyer, Constanze E. (2006-02)A recently published radiocarbon calibration curve extending to 50,000 cal BP (Fairbanks et al. 2005) is purportedly superior to that generated by the IntCal working group beyond the end of the tree-ring data at 12,400 ...