Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh
Bernhard, Anne E.
MetadataShow full item record
Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the effects of long-term fertilization on AOA and AOB in the Great Sippewissett Marsh, Falmouth, MA, USA to address some of these questions. Sediment samples were collected from low and high marsh habitats in July 2009 from replicate plots that received low (LF), high (HF), and extra high (XF) levels of a mixed NPK fertilizer biweekly during the growing season since 1974. Additional untreated plots were included as controls (C). Terminal restriction fragment length polymorphism analysis of the amoA genes revealed distinct shifts in AOB communities related to fertilization treatment, but the response patterns of AOA were less consistent. Four AOB operational taxonomic units (OTUs) predictably and significantly responded to fertilization, but only one AOA OTU showed a significant pattern. Betaproteobacterial amoA gene sequences within the Nitrosospira-like cluster dominated at C and LF sites, while sequences related to Nitrosomonas spp. dominated at HF and XF sites. We identified some clusters of AOA sequences recovered primarily from high fertilization regimes, but other clusters consisted of sequences recovered from all fertilization treatments, suggesting greater physiological diversity. Surprisingly, fertilization appeared to have little impact on abundance of AOA or AOB. In summary, our data reveal striking patterns for AOA and AOB in response to long-term fertilization, and also suggest a missing link between community composition and abundance and nitrogen processing in the marsh.
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Aquatic Microbiology 3 (2013): 445, doi:10.3389/fmicb.2012.00445.
The following license files are associated with this item:
Showing items related by title, author, creator and subject.
Plant nitrogen dynamics in fertilized and natural New England salt marshes : a paired 15N tracer study Drake, Deanne C.; Peterson, Bruce J.; Deegan, Linda A.; Harris, Lora A.; Miller, E. E.; Warren, R. Scott (Inter-Research, 2008-02-07)We examined the effects of increased nutrient availability on nitrogen (N) dynamics in dominant New England salt marsh plants (tall and stunted Spartina alterniflora and S. patens) using paired large-scale nutrient and ...
Distribution of hydrocarbons in a salt marsh ecosystem after an oil spill and physiological changes in marsh animals from the polluted environment Burns, Kathryn A. (Massachusetts Institute of Technology and Woods Hole Oceanographic Institution, 1975-06)The studies described in this thesis were designed to answer several problems relating to the recovery of a salt marsh heavily polluted by an accidental spill of Number 2 fuel oil. Field and laboratory studies were ...
Forbrich, Inke; Giblin, Anne E. (John Wiley & Sons, 2015-09-29)We studied marsh-atmosphere exchange of carbon dioxide in a high marsh dominated salt marsh during the months of May to October in 2012–2014. Tidal inundation at the site occurred only during biweekly spring tides, during ...