Probing the function of neuronal populations : combining micromirror-based optogenetic photostimulation with voltage-sensitive dye imaging
Kee, Michelle Z. L.
Loew, Leslie M.
Augustine, George J.
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KeywordOptogenetics; Channelrhodopsin; Digital micromirror device; Voltage-sensitive dye imaging; Inhibitory circuitry; Cerebellum
Recent advances in our understanding of brain function have come from using light to either control or image neuronal activity. Here we describe an approach that combines both techniques: a micromirror array is used to photostimulate populations of presynaptic neurons expressing channelrhodopsin-2, while a red-shifted voltage-sensitive dye allows optical detection of resulting postsynaptic activity. Such technology allowed us to control the activity of cerebellar interneurons while simultaneously recording inhibitory responses in multiple Purkinje neurons, their postsynaptic targets. This approach should substantially accelerate our understanding of information processing by populations of neurons within brain circuits.
Author Posting. © The Author(s), 2012. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Neuroscience Research 75 (2013): 76-81, doi:10.1016/j.neures.2012.11.006.
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