Comparison of techniques used to count single-celled viable phytoplankton
Steinberg, Mia K.
First, Matthew R.
Lemieux, Edward J.
Drake, Lisa A.
Nelson, Bruce N.
Kulis, David M.
Anderson, Donald M.
Welschmeyer, Nicholas A.
Herring, Penny R.
MetadataShow full item record
KeywordPhytoplankton; Enumeration; FlowCAM; Flow cytometry; Sedgewick Rafter; Ballast water; SYTOX Green; CellTracker Green
Four methods commonly used to count phytoplankton were evaluated based upon the precision of concentration estimates: Sedgewick Rafter and membrane filter direct counts, flow cytometry, and flow-based imaging cytometry (FlowCAM). Counting methods were all able to estimate the cell concentrations, categorize cells into size classes, and determine cell viability using fluorescent probes. These criteria are essential to determine whether discharged ballast water complies with international standards that limit the concentration of viable planktonic organisms based on size class. Samples containing unknown concentrations of live and UV-inactivated phytoflagellates (Tetraselmis impellucida) were formulated to have low concentrations (<100 ml-1) of viable phytoplankton. All count methods used chlorophyll a fluorescence to detect cells and SYTOX fluorescence to detect non-viable cells. With the exception of one sample, the methods generated live and non-viable cell counts that were significantly different from each other, although estimates were generally within 100% of the ensemble mean of all subsamples from all methods. Overall, percent coefficient of variation (CV) among sample replicates was lowest in membrane filtration sample replicates, and CVs for all four counting methods were usually lower than 30% (although instances of ~60% were observed). Since all four methods were generally appropriate for monitoring discharged ballast water, ancillary considerations (e.g., ease of analysis, sample processing rate, sample size, etc.) become critical factors for choosing the optimal phytoplankton counting method.
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Springer for personal use, not for redistribution. The definitive version was published in Journal of Applied Phycology 24 (2012): 751-758, doi:10.1007/s10811-011-9694-z.
Showing items related by title, author, creator and subject.
Palmitoylation regulates glutamate receptor distributions in postsynaptic densities through control of PSD95 conformation and orientation Jeyifous, Okunola; Lin, Eric I.; Chen, Xiaobing; Antinone, Sarah E.; Mastro, Ryan; Drisdel, Renaldo; Reese, Thomas S.; Green, William N. (2016-08)PSD95 and SAP97 are homologous scaffold proteins with different N-terminal domains, possessing either a palmitoylation site (PSD95) or an L27 domain (SAP97). Here, we measured PSD95 and SAP97 conformation in vitro and ...
Blaser, Martin J.; Cardon, Zoe G.; Cho, Mildred K.; Dangl, Jeffery; Donohue, Timothy J.; Green, Jessica L.; Knight, Rob; Maxon, Mary E.; Northen, Trent R.; Pollard, Katherine; Brodie, Eoin L. (American Society for Microbiology, 2016-05-13)Microorganisms have shaped our planet and its inhabitants for over 3.5 billion years. Humankind has had a profound influence on the biosphere, manifested as global climate and land use changes, and extensive urbanization ...
Sediment and nutrient delivery from thermokarst features in the foothills of the North Slope, Alaska : potential impacts on headwater stream ecosystems Bowden, W. B.; Gooseff, Michael N.; Balser, A.; Green, A.; Peterson, Bruce J.; Bradford, J. (American Geophysical Union, 2008-06-03)Permafrost is a defining characteristic of the Arctic environment. However, climate warming is thawing permafrost in many areas leading to failures in soil structure called thermokarst. An extensive survey of a 600 km2 ...