Spatial manipulation of cells and organelles using single electrode dielectrophoresis
MetadataShow full item record
The selection, isolation, and accurate positioning of single cells in three dimensions are increasingly desirable in many areas of cell biology and tissue engineering. We describe the application of a simple and low cost dielectrophoretic device for picking out and relocating single target cells. The device consists of a single metal electrode and an AC signal generator. It does not require microfabrication technologies or sophisticated electronics. The dielectrophoretic manipulator also discriminates between live and dead cells and is capable of redistributing intracellular organelles.
Author Posting. © Author(s), 2012. This article is posted here by permission of Informa Healthcare USA, for personal use, not for redistribution. The definitive version was published in BioTechniques 52 (2012): 39-43, doi:10.2144/000113802.
Showing items related by title, author, creator and subject.
The amyloid precursor protein of Alzheimer’s disease clusters at the organelle/microtubule interface on organelles that bind microtubules in an ATP dependent manner Stevenson, James W.; Conaty, Eliza A.; Walsh, Rylie B.; Poidomani, Paul J.; Samoriski, Colin M.; Scollins, Brianne J.; DeGiorgis, Joseph A. (Public Library of Science, 2016-01-27)The amyloid precursor protein (APP) is a causal agent in the pathogenesis of Alzheimer’s disease and is a transmembrane protein that associates with membrane-limited organelles. APP has been shown to co-purify through ...
Rediscovery of the nucleolinus, a dynamic RNA-rich organelle associated with the nucleolus, spindle, and centrosomes Alliegro, Mary Anne; Henry, Jonathan J.; Alliegro, Mark C. (2010-06)The nucleolinus is an RNA-rich compartment, closely apposed to or embedded within the nucleolus. Discovered over 150 years ago, fewer than two dozen articles have been published on the nucleolinus, probably because complex ...
Johnson, Matthew D. (2010-04)Many non-photosynthetic species of protists and metazoans are capable of hosting viable algal endosymbionts or their organelles through adaptations of phagocytic pathways. A form of mixotrophy, acquired phototrophy (AcPh) ...