Diversity of Archaea and detection of crenarchaeotal amoA genes in the rivers Rhine and Têt
Coolen, Marco J. L.
Herndl, Gerhard J.
Sinninghe Damste, Jaap S.
MetadataShow full item record
Pelagic archaeal phylogenetic diversity and the potential for crenarchaeotal nitrification of Group 1.1a were determined in the rivers Rhine and Têt by 16S rRNA sequencing, catalyzed reported deposition-fluorescence in situ hybridization (CARD–FISH) and quantification of 16S rRNA and functional genes. Euryarchaeota were, for the first time, detected in temperate river water even though a net predominance of crenarchaeotal phylotypes was found. Differences in phylogenic distribution were observed between rivers and seasons. Our data suggest that a few archaeal phylotypes (Euryarchaeota Groups RC-V and LDS, Crenarchaeota Group 1.1a) are widely distributed in pelagic riverine environments whilst others (Euryarchaeota Cluster Sagma-1) may only occur seasonally in river water. Crenarchaeota Group 1.1a has recently been identified as a major nitrifier in the marine environment and phylotypes of this group were also present in both rivers, where they represented 0.3% of the total pelagic microbial community. Interestingly, a generally higher abundance of Crenarchaeota Group 1.1a was found in the Rhine than in the Têt, and crenarchaeotal ammonia monooxygenase gene (amoA) was also detected in the Rhine, with higher amoA copy numbers measured in February than in September. This suggests that some of the Crenarchaeota present in river waters have the ability to oxidize ammonia and that riverine crenarchaeotal nitrification of Group 1.1a may vary seasonally.
Author Posting. © Inter-Research, 2009. This article is posted here by permission of Inter-Research for personal use, not for redistribution. The definitive version was published in Aquatic Microbial Ecology 55 (2009): 189-201, doi:10.3354/ame01294.
Showing items related by title, author, creator and subject.
Genomic and transcriptomic evidence for scavenging of diverse organic compounds by widespread deep-sea archaea Li, Meng; Baker, Brett J.; Anantharaman, Karthik; Jain, Sunit; Breier, John A.; Dick, Gregory J. (Nature Publishing Group, 2015-11-17)Microbial activity is one of the most important processes to mediate the flux of organic carbon from the ocean surface to the seafloor. However, little is known about the microorganisms that underpin this key step of the ...
Differential responses of ammonia-oxidizing archaea and bacteria to long-term fertilization in a New England salt marsh Peng, Xuefeng; Yando, Erik; Hildebrand, Erica; Dwyer, Courtney; Kearney, Anne; Waciega, Alex; Valiela, Ivan; Bernhard, Anne E. (Frontiers Media, 2013-01-22)Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the ...
Archaea and bacteria with surprising microdiversity show shifts in dominance over 1,000-year time scales in hydrothermal chimneys Brazelton, William J.; Ludwig, Kristin A.; Sogin, Mitchell L.; Andreishcheva, Ekaterina N.; Kelley, Deborah S.; Shen, Chuan-Chou; Edwards, R. Lawrence; Baross, John A. (2009-12-06)The Lost City Hydrothermal Field, an ultramafic-hosted system located 15 km west of the Mid-Atlantic Ridge, has experienced at least 30,000 years of hydrothermal activity. Previous studies have shown that its carbonate ...