Transcriptome analyses of the Giardia lamblia life cycle
Birkeland, Shanda R.
Preheim, Sarah P.
Davids, Barbara J.
Cipriano, Michael J.
Reiner, David S.
Svard, Staffan G.
Gillin, Frances D.
McArthur, Andrew G.
MetadataShow full item record
We quantified mRNA abundance from 10 stages in the Giardia lamblia life cycle in vitro using Serial Analysis of Gene Expression (SAGE). 163 abundant transcripts were expressed constitutively. 71 transcripts were upregulated specifically during excystation and 42 during encystation. Nonetheless, the transcriptomes of cysts and trophozoites showed major differences. SAGE detected co-expressed clusters of 284 transcripts differentially expressed in cysts and excyzoites and 287 transcripts in vegetative trophozoites and encysting cells. All clusters included known genes and pathways as well as proteins unique to Giardia or diplomonads. SAGE analysis of the Giardia life cycle identified a number of kinases, phosphatases, and DNA replication proteins involved in excystation and encystation, which could be important for examining the roles of cell signaling in giardial differentiation. Overall, these data pave the way for directed gene discovery and a better understanding of the biology of Giardia lamblia.
Author Posting. © The Author(s), 2010. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Molecular and Biochemical Parasitology 174 (2010): 62-65, doi:10.1016/j.molbiopara.2010.05.010.
Showing items related by title, author, creator and subject.
The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP) : illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing Keeling, Patrick J.; Burki, Fabien; Wilcox, Heather M.; Allam, Bassem; Allen, Eric E.; Amaral-Zettler, Linda A.; Armbrust, E. Virginia; Archibald, John M.; Bharti, Arvind K.; Bell, Callum J.; Beszteri, Bank; Bidle, Kay D.; Cameron, Connor T.; Campbell, Lisa; Caron, David A.; Cattolico, Rose Ann; Collier, Jackie L.; Coyne, Kathryn J.; Davy, Simon K.; Deschamps, Phillipe; Dyhrman, Sonya T.; Edvardsen, Bente; Gates, Ruth D.; Gobler, Christopher J.; Greenwood, Spencer J.; Guida, Stephanie M.; Jacobi, Jennifer L.; Jakobsen, Kjetill S.; James, Erick R.; Jenkins, Bethany D.; John, Uwe; Johnson, Matthew D.; Juhl, Andrew R.; Kamp, Anja; Katz, Laura A.; Kiene, Ronald P.; Kudryavtsev, Alexander N.; Leander, Brian S.; Lin, Senjie; Lovejoy, Connie; Lynn, Denis; Marchetti, Adrian; McManus, George; Nedelcu, Aurora M.; Menden-Deuer, Susanne; Miceli, Cristina; Mock, Thomas; Montresor, Marina; Moran, Mary Ann; Murray, Shauna A.; Nadathur, Govind; Nagai, Satoshi; Ngam, Peter B.; Palenik, Brian; Pawlowski, Jan; Petroni, Giulio; Piganeau, Gwenael; Posewitz, Matthew C.; Rengefors, Karin; Romano, Giovanna; Rumpho, Mary E.; Rynearson, Tatiana A.; Schilling, Kelly B.; Schroeder, Declan C.; Simpson, Alastair G. B.; Slamovits, Claudio H.; Smith, David R.; Smith, G. Jason; Smith, Sarah R.; Sosik, Heidi M.; Stief, Peter; Theriot, Edward; Twary, Scott N.; Umale, Pooja E.; Vaulot, Daniel; Wawrik, Boris; Wheeler, Glen L.; Wilson, William H.; Xu, Yan; Zingone, Adriana; Worden, Alexandra Z. (Public Library of Science, 2014-06-24)Microbial ecology is plagued by problems of an abstract nature. Cell sizes are so small and population sizes so large that both are virtually incomprehensible. Niches are so far from our everyday experience as to make their ...
Transcriptome data reveal syndermatan relationships and suggest the evolution of endoparasitism in Acanthocephala via an epizoic stage Wey-Fabrizius, Alexandra R.; Herlyn, Holger; Rieger, Benjamin; Rosenkranz, David; Witek, Alexander; Mark Welch, David B.; Ebersberger, Ingo; Hankeln, Thomas (Public Library of Science, 2014-02-10)The taxon Syndermata comprises the biologically interesting wheel animals (“Rotifera”: Bdelloidea + Monogononta + Seisonidea) and thorny-headed worms (Acanthocephala), and is central for testing superordinate phylogenetic ...
Comparative transcriptome analysis of obligately asexual and cyclically sexual rotifers reveals genes with putative functions in sexual reproduction, dormancy, and asexual egg production Hanson, Sara J.; Stelzer, Claus-Peter; Mark Welch, David B.; Logsdon, John M. (BioMed Central, 2013-06-19)Sexual reproduction is a widely studied biological process because it is critically important to the genetics, evolution, and ecology of eukaryotes. Despite decades of study on this topic, no comprehensive explanation has ...