Proteomic identification, cDNA cloning and enzymatic activity of glutathione S-transferases from the generalist marine gastropod, Cyphoma gibbosum
Whalen, Kristen E.
Lin, Ching Yu
Tjeerdema, Ronald S.
Goldstone, Jared V.
Hahn, Mark E.
MetadataShow full item record
KeywordCyphoma gibbosum; Glutathione S-transferase; Gorgonian; Natural product; Chemical ecology; Allelochemical; Proteomic
Glutathione S-transferases (GST) were characterized from the digestive gland of Cyphoma gibbosum (Mollusca; Gastropoda), to investigate the possible role of these detoxification enzymes in conferring resistance to allelochemicals present in its gorgonian coral diet. We identified the collection of expressed cytosolic Cyphoma GST classes using a proteomic approach involving affinity chromatography, HPLC and nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS). Two major GST subunits were identified as putative mu-class GSTs; while one minor GST subunit was identified as a putative theta-class GST, apparently the first theta-class GST identified from a mollusc. Two Cyphoma GST cDNAs (CgGSTM1 and CgGSTM2) were isolated by RT-PCR using primers derived from peptide sequences. Phylogenetic analyses established both cDNAs as mu-class GSTs and revealed a mollusc-specific subclass of the GST-mu clade. These results provide new insights into metazoan GST diversity and the biochemical mechanisms used by marine organisms to cope with their chemically defended prey.
Author Posting. © Elsevier B.V., 2008. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Archives of Biochemistry and Biophysics 478 (2008): 7-17, doi:10.1016/j.abb.2008.07.007.
Showing items related by title, author, creator and subject.
Environmental sensing and response genes in cnidaria : the chemical defensome in the sea anemone Nematostella vectensis Goldstone, Jared V. (2008-10)The starlet sea anemone Nematostella vectensis has been recently established as a new model system for the study of the evolution of developmental processes, as cnidaria occupy a key evolutionary position at the base of ...
Modelling interactions of acid–base balance and respiratory status in the toxicity of metal mixtures in the American oyster Crassostrea virginica Macey, Brett M.; Jenny, Matthew J.; Williams, Heidi R.; Thibodeaux, Lindy K.; Beal, Marion; Almeida, Jonas S.; Cunningham, Charles; Mancia, Annalaura; Warr, Gregory W.; Burge, Erin J.; Holland, A. Fredrick; Gross, Paul S.; Hikima, Sonomi; Burnett, Karen G.; Burnett, Louis; Chapman, Robert W. (2009-11-12)Heavy metals, such as copper, zinc and cadmium, represent some of the most common and serious pollutants in coastal estuaries. In the present study, we used a combination of linear and artificial neural network (ANN) ...
Timme-Laragy, Alicia R.; Van Tiem, Lindsey A.; Linney, Elwood A.; Di Giulio, Richard T. (2008-12)Early piscine life-stages are sensitive to polycyclic aromatic hydrocarbon (PAH) exposure, which can cause pericardial effusion and craniofacial malformations. We previously reported that certain combinations of PAHs ...