Characterization of protistan assemblages in the Ross Sea, Antarctica, by denaturing gradient gel electrophoresis
MetadataShow full item record
The diversity of protistan assemblages has traditionally been studied using microscopy and morphological characterization, but these methods are often inadequate for ecological studies of these communities because most small protists inherently lack adequate taxonomic characters to facilitate their identification at the species level and many protistan species also do not preserve well. We have therefore used a culture-independent approach (denaturing gradient gel electrophoresis [DGGE]) to obtain an assessment of the genetic composition and distribution of protists within different microhabitats (seawater, meltwater or slush on sea-ice floes, and ice) of the Ross Sea, Antarctica. Samples of the same type (e.g., water) shared more of the same bands than samples of different types (e.g., ice versus water), despite being collected from different sites. These findings imply that samples from the same environment have a similar protistan species composition and that the type of microenvironment significantly influences the protistan species composition of these Antarctic assemblages. It should be noted that a large number of bands among the samples within each microhabitat were distinct, indicating the potential presence of significant genetic diversity within each microenvironment. Sequence analysis of selected DGGE bands revealed sequences that represent diatoms, dinoflagellates, ciliates, flagellates, and several unidentified eukaryotes.
Author Posting. © American Society for Microbiology, 2004. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 70 (2004): 2028-2037, doi:10.1128/AEM.70.4.2028-2037.2004.
Showing items related by title, author, creator and subject.
A method for studying protistan diversity using massively parallel sequencing of V9 hypervariable regions of small-subunit ribosomal RNA genes Amaral-Zettler, Linda A.; McCliment, Elizabeth A.; Ducklow, Hugh W.; Huse, Susan M. (Public Library of Science, 2009-07-27)Massively parallel pyrosequencing of amplicons from the V6 hypervariable regions of small-subunit (SSU) ribosomal RNA (rRNA) genes is commonly used to assess diversity and richness in bacterial and archaeal populations. ...
Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea Edgcomb, Virginia P.; Orsi, William D.; Leslin, Chesley; Epstein, Slava S.; Bunge, John; Jeon, Sunok; Yakimov, Michail M.; Behnke, Anke; Stoeck, Thorsten (2008-10-21)Environmental factors restrict the distribution of microbial eukaryotes but the exact boundaries for eukaryotic life are not known. Here we examine protistan communities at the extremes of salinity and osmotic pressure, ...
Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities Stoeck, Thorsten; Behnke, Anke; Christen, Richard; Amaral-Zettler, Linda A.; Rodriguez-Mora, Maria J.; Chistoserdov, Andrei Y.; Orsi, William D.; Edgcomb, Virginia P. (BioMed Central, 2009-11-03)Recent advances in sequencing strategies make possible unprecedented depth and scale of sampling for molecular detection of microbial diversity. Two major paradigm-shifting discoveries include the detection of bacterial ...