Readme Document describing raw sequence read files for the Nematostella developmental high-density RNAseq time course. 

to be published in: not clear yet

Nematostella High Density RNAseq Timecourse

created from Illumina HiSeq 100bp paired end reads.  

cDNA libraries and raw reads created by Antje Fischer, for questions contact: afischer@mbl.edu

All samples were collected in one big spawning event of several males and females. For each sample 300 embryos were counted and collected. Sampling was started with unfertilized eggs (0 hours (h)), every hour until 19h. For each time point a sample A and B were collected. Samples of the same time point were always collected within 10 min. The preparation of sample 10h B failed, thus there is only one sample for 10h, which is called 10h A.
The samples 3h A, 4h A, 5h B, 6h B, 7h A, 8h B and 9h B might have been randomly swapped among each other during the library preparation procedure. The exact stage is therefore not known for these samples. 

Example file name: 
2h_B_GGCTAC_L004_R1_003
2h: time point of collection, 2h after fertilization
_B: sample B
_GGCTAC: The 6-letter code in the file name represents the index used.
_L004: the sample was sequenced in lane 4; each sample was split on two lanes to avoid lane specific effects
_R1: Read 1; R1 and R2 correspond to Read 1 and Read 2 for paired end reads. 
_003: data pack 003; each dataset was subdivided into four to six files to ensure individual files can be managed. 

Files contain the sequences for individual stages before removal of ribosomal sequences and removal of poly-A sequences.